TY - JOUR AU - Shiraki, Yutaka AU - Shibata, Naohiro AU - Doi, Yohei AU - Arakawa, Yoshichika T1 - Escherichia coli Producing CTX-M-2 β-Lactamase in Cattle, Japan T2 - Emerging Infectious Disease journal PY - 2004 VL - 10 IS - 1 SP - 69 SN - 1080-6059 AB - From November 2000 to June 2001, Escherichia coli strains producing CTX-M-2 β-lactamase were isolated from 6 (1.5%) of 396 cattle fecal samples and 2 (0.7%) of 270 surface swabs of cattle carcasses in Japan. The blaCTX-M-2 gene responsible for CTX-M-2 production was encoded on transferable plasmids, and the gene was transferred to E. coli CSH2 with a very high frequency (2 x 10-4 to 6 x 10-1 per donor cells) by conjugation. Random amplified polymorphic DNA analysis of nine isolates showed at least five different patterns. These findings suggest that CTX-M-2 producers might have originated from cattle through the use of cephalosporins such as ceftiofur and that cattle could be a reservoir of CTX-M-2–producing E. coli. Continuous and strategic surveillance of antimicrobial-resistant bacteria in livestock is essential to suppress further dissemination of these bacteria into society at large. KW - CTX-M-type beta-lactamase KW - extended-spectrum beta-lactamase KW - Escherichia coli KW - random amplified polymorphic DNA KW - cattle KW - livestock KW - slaughterhouse KW - antimicrobial-resistance KW - surveillance KW - Japan DO - 10.3201/eid1001.030219 UR - https://wwwnc.cdc.gov/eid/article/10/1/03-0219_article ER - End of Reference