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Volume 10, Number 10—October 2004
Research

Escherichia coli and Community-acquired Gastroenteritis, Melbourne, Australia

Roy M. Robins-Browne*†Comments to Author , Anne-Marie Bordun*†, Marija Tauschek*, Vicki R. Bennett-Wood*†, Jacinta Russell†, Frances Oppedisano†, Nicole A. Lister*, Karl A. Bettelheim*, Christopher K. Fairley*, Martha I. Sinclair‡, and Margaret E. Hellard‡1
Author affiliations: *University of Melbourne, Melbourne, Victoria, Australia; †Murdoch Childrens Research Institute, Melbourne, Victoria, Australia; ‡Monash University, Melbourne, Victoria, Australia

Main Article

Figure 3

Fluorescent actin staining (FAS) assay for attaching-effacing capacity of atypical enteropathogenic Escherichia coli (EPEC) strains with different patterns of adherence to HEp-2 cells. Fluorescent micrographs of HEp-2 cells (A and B) incubated with strains of atypical EPEC showing localized-like and aggregative adherence, respectively, and then reacted with fluorescein-labelled phalloidin. Note the foci of intense fluorescence (arrows) associated with adherent bacteria, which were also visualize

Figure 3. Fluorescent actin staining (FAS) assay for attaching-effacing capacity of atypical enteropathogenic Escherichia coli (EPEC) strains with different patterns of adherence to HEp-2 cells. Fluorescent micrographs of HEp-2 cells (A and B) incubated with strains of atypical EPEC showing localized-like and aggregative adherence, respectively, and then reacted with fluorescein-labelled phalloidin. Note the foci of intense fluorescence (arrows) associated with adherent bacteria, which were also visualized by phase contrast microscopy of the same microscope fields (C and D). Magnification x1,000.

Main Article

1Current affiliation: Burnet Institute, Melbourne, Victoria, Australia.

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