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Volume 10, Number 12—December 2004

Dispatch

Antibodies to SARS-Coronavirus in Civets

Changchun Tu*1, Gary Crameri†1, Xiangang Kong‡, Jinding Chen§, Yanwei Sun¶, Meng Yu†, Hua Xiang*, Xianzhu Xia*, Shengwang Liu‡, Tao Ren§, Yedong Yu¶, Bryan T. Eaton†, Hua Xuan*Comments to Author , and Lin-Fa Wang†
Author affiliations: *Changchun University of Agriculture and Animal Sciences, Changchun, China; †CSIRO Livestock Industries, Geelong, Australia; ‡Harbin Veterinary Research Institute, Harbin, China; §South China Agriculture University, Guangzhou; China; ¶Guangdong Provincial Veterinary Station of Epidemic Prevention and Supervision, Guangzhou, China

Main Article

Table

Summary of serologic analyses of civet serum samplesa

Sample no. Farm
Market
Hunan
Henan
Guangdong
Guangdong
Changsha
Luoning
Qianguan
Shaoguan
Shanwei
Zhuhai
Guangzhou
VNTb IFAc VNTb IFAc VNTb IFAc VNTb IFAc VNTb IFAc WBd VNTb IFAc VNTb IFAc WBd
1 NA NA 10 10 +++ ++++
2 10 + + 640 ++ ++++
3 NA NA CONT +++ +++
4 20 +++ ++++
5 40 +/– +
6 20 30 ++ +++
7
8 10 ++ +++
9 +
10
11 10 ++ ++++
12 NA NA NA
13 20 ++ ++++
14 30 ++ ++++
15 NA NA NA
16 10 ++ ++++
17 10 +++ ++++
18 10 ++ ++++
19 240 +++ ++++
20 60 +++ ++++
21
22
23
24
25
26
27
28
29
30

a VNT, virus neutralization test; IFA, immunofluorescent antibody test; WB, Western blot test; NA, not assayed because of sample damage or loss during transportation or storage; CONT, contaminated samples that exhibited toxicity in VNT test.
bTiter of antibody that neutralized infectivity of 200 50% tissue culture infective dose of SARS-CoV.
cImmunofluorescent antibody test was performed by using a serum dilution of 1:50, and the intensity of staining in SARS-CoV–infected cells, but not in mock infected cells, was indicated by the sign +, with +++ representing the strongest signal observed.
dWestern blot was conducted by using a serum dilution of 1:50, and the reactivity with the recombinant N protein expressed was indicated by the sign +, with ++++ representing the strongest signal observed.

Main Article

1These authors contributed equally to this study.

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