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Volume 10, Number 12—December 2004

Research

Cats as a Risk for Transmission of Antimicrobial drug−resistant Salmonella

Filip Van Immerseel*Comments to Author , Frank Pasmans*, Jeroen De Buck*, Ivan Rychlik†, Helena Hradecka†, Jean-Marc Collard‡, Christa Wildemauwe§, Marc Heyndrickx¶, Richard Ducatelle*, and Freddy Haesebrouck*
Author affiliations: *Ghent University, Merelbeke, Belgium; †Veterinary Research Institute, Brno, Czech Republic; ‡Scientific Institute of Public Health, Brussels, Belgium; §Pasteur Institute of Brussels, Brussels, Belgium; ¶Center for Agricultural Research, Melle, Belgium.

Main Article

Table 1

List of primers used in the PCR reactions for detection of resistance genes

Resistance Gene Primer Sequence (5′– 3′) Size (bp) Reference
Ampicillin blaPSE­1 PSEF TAG CCA TAT TAT GGA GCC TC 321 AF261825
PSER TTA ACT TTT CCT TGC TCA GC
blaTEM TEMF GCA CGA GTG GGT TAC ATC GA 310 9
TEMR GGT CCT CCG ATC GTT GTC AG
blaoxa1 oxa1F AGC AGC GCC AGT GCA TCA 708 10


oxa1R
ATT CGA CCC CAA GTT TCC


Chloramphenicol floR floRF GCG ATA TTC ATT ACT TTG GC 425 11
floRR TAG GAT GAA GGT GAG GAA TG
Cat catF CCT GCC ACT CAT CGC AGT 623 10


catR
CCA CCG TTG ATA TAT CCC


Streptomycin aadA1 aad1For CGA CTC AAC TAT CAG AGG TA 384 AY534545
aad1Rev CTT TTG TCA GCA AGA TAG CC
aadA2 aadA2F CGG TGA CCA TCG AAA TTT CG 249 12
aadA2R CTA TAG CGC GGA GCG TCT CGC
strA strAF CCT ATC GGT TGA TCA ATG TC 250 11


strAR
GAA GAG TTT TAG GGT CCA CC


Tetracycline tetA tetAF GCT ACA TCC TGC TTG CCT TC 210 13
tetAR CAT AGA TCG CCG TGA AGA GG
tetB tetBF TTG GTT AGG GGC AAG TTT TG 659 13
tetBR GTA ATG GGC CAA TAA CAC CG
tetC tetCF GCG GGA TAT CGT CCA TTC CG 207 14
tetCR GCG TAG AGG ATC CAC AGG ACG
tetG tetGF GCT CGG TGG TAT CTC TGC TC 468 13


tetGR
AGC AAC AGA ATC GGG AAC AC


Sulfonamide sul1 sul1F ATG GTG ACG GTG TTC GGC ATT CTG 841 15
sul1R GCT AGG CAT GAT CTA ACC CTC GG
sul2 sul2F AGG GGG CAG ATG TGA TCG AC 249 11


sul2R
GCA GAT GAT TTC GCC AAT TG


Trimethoprim dfrA1 dfrA1F GTG AAA CTA TCA CTA ATG G 470 10
dfrA1R CCC TTT TGC CAG ATT TGG
dfrA10 dfrA10F TTA ATT ACC AGA GCA TTC GG 374 AY049746
dfrA10R TAC ACA TCA GCA TGA ACA GG
dfrA12 dfrA12F ACT CGG AAT CAG TAC GCA 463 10


dfrA12R
GTG TAC GGA ATT ACA GCT


Kanamycin aadD aadD¬F ATA TTG GAT AAA TAT GGG GAT 161 12
aadD¬R TCC ACC TTC CAC TCA CCG GTT
aphA/aph(3′)¬Id aphAaphIdF ATG GGC GCC TAT CAC AAT TGG 257 12
aphAaphIdR TCG CCT CCA GCT CTT CGT AGA
aphAI¬IAB aphAI¬IABF AAA CGT CTT GCT CGA GGC 461 12
aphAI¬IABR CAA ACC GTT ATT CAT TCG TGA
aph(3′)¬IIa KanAphF GAG AAA GTA TCC ATC ATG GC 465 L19385
KanAphR GCT CAG AAG AAC TCG TCA AG

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