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Volume 10, Number 2—February 2004
THEME ISSUE
2004 SARS Edition

Laboratory Study

Ultrastructural Characterization of SARS Coronavirus

Cynthia S. Goldsmith*, Kathleen M. Tatti*, Thomas G. Ksiazek*, Pierre E. Rollin*, James A. Comer*, William W. Lee*, Paul A. Rota*, Bettina Bankamp*, William J. Bellini*, and Sherif R. Zaki*
Author affiliations: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA

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Figure 2

Detection of viral proteins and viral RNA associated with intracytoplasmic virions. A) Immunogold labeling of viral proteins by using hyperimmune mouse ascitic fluid directed against severe acute respiratory syndrome–associated coronavirus (12 nm gold). B) Ultrastructural in situ hybridization detection of viral RNA by using a pool of polymerase and nucleocapsid riboprobes (6 nm gold). Bars, 100 nm.

Figure 2. Detection of viral proteins and viral RNA associated with intracytoplasmic virions. A) Immunogold labeling of viral proteins by using hyperimmune mouse ascitic fluid directed against severe acute respiratory syndrome–associated coronavirus (12 nm gold). B) Ultrastructural in situ hybridization detection of viral RNA by using a pool of polymerase and nucleocapsid riboprobes (6 nm gold). Bars, 100 nm.

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