Volume 10, Number 4—April 2004
Research
Antimicrobial Resistance Gene Delivery in Animal Feeds
Karen Lu*, Rumi Asano†, and Julian Davies*
Table
Primer sequences for genes vanH, ddlN, vanX and partial 16S rDNA
| Primer | Primer sequences |
|---|---|
| vanH-1 | 5′-CAC ATC GA(C/T) GTG GAA TAC GC-3′ |
| vanH-2 | 5′-CAG TCG GCG TAG AAG ATG CC-3′ |
| vanH-3 | 5′-GAG GAA GGC ATC TTC TAC GC-3′ |
| ddlN-1 | 5′-ACG (G/C)CA GTA CGA C(G/T)C GAA G-3′ |
| ddlN-2 | 5′-T(G/T)C CTG GA(A/T) GCT (G/C)TG CGA C-3′ |
| ddlN-3 | 5′-G(A/G)T AAC GGC TGT ACG AGG TC-3′ |
| vanX-3 | 5′-CCA CGT GGG ACA ACT TCA C-3′ |
| vanX-4 | 5′-CAG (C/G)(G/T)T GTA GTG CCA CCA CTC-3′ |
| vanX-5 | 5-TCA CCA GAT ATC CGT CCA CC-3′ |
| 16S 440F | 5′-AGC AGG GAA GAA GCG (A/T/C)(A/G)A GT-3′ |
| 16S 1491R | 5′-CGG CTA CCT TGT TAC GAC TTC-3′ |
