Volume 10, Number 5—May 2004
Research
Acute Tick-borne Rickettsiosis Caused by Rickettsia heilongjiangensis in the Russian Far East
Oleg Y. Mediannikov*†‡
, Yuri Sidelnikov†, Leonid Ivanov§, Eugenia Mokretsova†, Pierre-Edouard Fournier‡, Irina Tarasevich*, and Didier Raoult‡
, Yuri Sidelnikov†, Leonid Ivanov§, Eugenia Mokretsova†, Pierre-Edouard Fournier‡, Irina Tarasevich*, and Didier Raoult‡Table 1
List of the primers used to detect rickettsial DNAa
| Gene for amplification | Primer name | Primer sequence 5´-3´a | Annealing temperature |
|---|---|---|---|
| gltA | CS1d | ATGACTAATGGCAATAATAA | |
| CS2d | ATGACCAATGAAAATAATAAT | 50°C | |
| CSEndr | CTTATACTCTCTATGTACA | ||
| RpCS877p | GGGGACCTGCTCACGGCGG | 54°C | |
| RpCS1258n | ATTGCAAAAAGTACAGTGAACA | ||
| ompA, 5´- portion | 190-70 | ATGGCGAATATTTCTCCAAAA | 53°C |
| 190-701 | GTTCCGTTAATGGCAGCATCT | ||
| ompA, 3´- portion | 190-3588f | AACAGTGAATGTAGGAGCAG | 46°C for the first round 50°C for the nested and heminested rounds |
| 190-3968r | TAGCAGCTGATTTAGTAGCT | ||
| 190-4084f | CATCACCGATATTTCTAGC | ||
| 190-4338f | TTCAGGAAACGACCGTACG | ||
| 190-4406r | ACTATACCCTCATCGTCATT | ||
| 190-4859f | GCGAAATCCAAGGTACAGG | ||
| 190-5044r | AACTTGTAGCACCTGCCGT | ||
| 190-5125f | GCGGTTACTTTAGCCAAAGG | ||
| 190-5238r | ACTATTAAAGGCTAGGCTATT | ||
| 190-5768f | CACCGCTACAGGAAGCAGAT | ||
| 190-5831r | GTGTCGCTAGGTTTTACAAC | ||
| 190-6228f | CGTTGAAGTATAGCATC | ||
| 190-6427r | ATCTAAGCCCAGCTAGCGGT | ||
| 190-6808r | CACGAACTTTCACACTACC | ||
| ompB |
120-M36 |
TTCTACAGCTACCATAGTAGCCA |
50°C |
| 120-607 | AATATCGGTGACGGTCAAGG | ||
| 120-807 | CCTTTTAGATTACCGCCTAA | ||
| 120-1378 | TAAACTTGCTGACGGTACAG | ||
| 120-1497 | CCTATATCGCCGGTAATT | ||
| 120-2113 | CGATGCTAACGTAGGTTCTT | ||
| 120-2339 | CTTGTTTGTTTAATGTTACGGT | ||
| 120-2778 | AAACAATAATCAAGGTACTGT | ||
| 120-2988 | CCGGCTATACCGCCTGTAGT | ||
| 120-3462 | CCACAGGAACTACAACCATT | ||
| 120-3599 | TACTTCCGGTTACAGCAAAGT | ||
| 120-4232 | GGTTTCTCATTCTCTCTATATGG | ||
| 120-4346 | CGAAGAAGTAACGCTGACTT | ||
| 120-4879 | TTAGAAGTTTACACGGACTTTT |
aDifferences between primers CS1d and CS2d are indicated by bold letters.
