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Volume 10, Number 6—June 2004

Research

Candida parapsilosis Characterization in an Outbreak Setting

Duncan M. Kuhn*1, Pranab K. Mukherjee*, Thomas A. Clark†, Claude Pujol‡, Jyotsna Chandra*, Rana A. Hajjeh†, David W. Warnock†, David R. Soll‡, and Mahmoud A. Ghannoum*Comments to Author 
Author affiliations: *University Hospitals of Cleveland and Case Western Reserve University, Cleveland, Ohio, USA; †Centers for Disease Control and Prevention, Atlanta, Georgia, USA; ‡University of Iowa, Iowa City, Iowa, USA; 1Current affiliation: Division of Pulmonary and Critical Care, University of Washington Hospitals, Seattle, WA 98195, USA.

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Figure 5

Secretory aspartic protease (SAP) expression by Candida parapsilosis clinical isolates. Panel A shows representative sodium dodecyl sulfate–polyacrylamide gel electrophoresis of various C. parapsilosis isolates. M, molecular weight marker lane; BSA, bovine serum albumin alone; other lanes show number of isolate; and +, supernatant plus protease inhibitor cocktail. Protease activity is evident from the appearance of lower molecular weight bands representing cleavage products. Thick arrow indicate

Figure 5. Secretory aspartic protease (SAP) expression by Candida parapsilosis clinical isolates. Panel A shows representative sodium dodecyl sulfate–polyacrylamide gel electrophoresis of various C. parapsilosis isolates. M, molecular weight marker lane; BSA, bovine serum albumin alone; other lanes show number of isolate; and +, supernatant plus protease inhibitor cocktail. Protease activity is evident from the appearance of lower molecular weight bands representing cleavage products. Thick arrow indicates the 20-kDa protein appearing after protease digestion. (For details of methods used see text.) Panel B shows densitometric scanning analysis of SAP activity. Strains 177 and 179 were included to demonstrate the heterogeneity in SAP production within the clonal strains. Cath, catheter; Bld, bloodstream; Spt, sputum; Hnd, hand; Wnd, wound; Pdf, peritoneal dialysis fluid.

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