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Volume 10, Number 7—July 2004

Dispatch

SARS Coronavirus Detection

Andreas Nitsche*Comments to Author , Brunhilde Schweiger*, Heinz Ellerbrok*, Matthias Niedrig*, and Georg Pauli*
Author affiliations: *Robert Koch-Institut, Berlin, Germany

Main Article

Table 1

Primers and 5′-nuclease probes of the three SARS coronavirus–specific assaysa

Primer/probe Primer/probe sequence Oligonucleotide orientation Nucleotide positionb Tm (°C)c

NS pp1a (133 bp)d



pp1a F
GCCgTAgTgTCAgTATCATCACC
S
4609–4631
56.6
pp1a R
AATAggACCAATCTCTgTAAgAgCC
A
4741–4717
56.7
pp1a TM
F-TCACTTCgTCATCAAAgACATC XT gAggAgC p
S
4661–4690
66.2

NS pp1ab (88 bp)d



NS F
TTTTgTTgTTTCAACTggATACCAT
S
14387–411
57.0
NS R
GAAACTgAgACgCgAgCTATgT
A
14474–453
57.3
NS TM
F-CATCCTgATTATgTACgACTCCTAAC XT CACgAA p
A
14445–413
64.4

Surface spike glycoprotein (79 bp)d



SS GP F
gAggTCTTTTATTgAggACTTgCTC
S
23879–903
57.1
SS GP R
gCATTCgCCATATTgCTTCAT
A
23957–937
57.3
SS GP TM F-AAgCCAgCATCAgCgAgTgTCACCTTA XT p A 23935–908 66.7

aAbbreviations: SARS, severe acute respiratory syndrome; S, sense, A, antisense; F, 6-carboxyfluorescein attached to 5´-terminus (FAM); T, 5-carboxytetramethylrhodamine (5-TAMRA) attached to 5-ethylamino-dThymidin; NS, nonstructural; pp1ab, polyprotein 1ab gene; Tm, melting temperature; TM, TaqMan.
bBased on AY274119 isolate TOR2.
cThermodynamic Tm. All oligonucleotides were synthesized by TIB MOLBIOL Berlin.
dAmplicon length.

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