Detection of SARS-associated Coronavirus in Throat Wash and Saliva in Early Diagnosis
Wei-Kung Wang*†, Shey-Ying Chen†1, I-Jung Liu*1, Yee-Chun Chen†, Hui-Ling Chen*, Chao-Fu Yang*, Pei-Jer Chen*, Shiou-Hwei Yeh‡, Chuan-Liang Kao*, Li-Min Huang†, Po-Ren Hsueh†, Jann-Tay Wang†, Wang-Hwei Sheng†, Chi-Tai Fang†, Chien-Ching Hung†, Szu-Min Hsieh†, Chan-Ping Su†, Wen-Chu Chiang†, Jyh-Yuan Yang§, Jih-Hui Lin§, Szu-Chia Hsieh*, Hsien-Ping Hu*, Yu-Ping Chiang*, Jin-Town Wang*, Pan-Chyr Yang†, Shan-Chwen Chang† , and members of the SARS Research Group of the National Taiwan UniversityNational Taiwan University Hospital
Author affiliations: *National Taiwan University, Taipei, Taiwan; †National Taiwan University Hospital, Taipei, Taiwan; ‡National Health Research Institute, Taipei, Taiwan; §Center for Disease Control, Department of Health, Taipei, Taiwan; 1S.-Y. Chen and I-J. Liu contributed equally to the work.
Figure 1. Quantification of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) RNA by real-time reverse transcription–polymerase chain reaction (RT-PCR) assay. (A) Location of the forward and reverse primers and probe in the genome of SARS-CoV, with the genome positions shown according to the Urbani strain (20). (B) A schematic diagram of the construct, ORF1b/pCRII-TOPO, and the protocol for generating the in vitro transcribed RNA as the standard for the real-time RT-PCR assay is shown. The relationship between known input RNA copies to the threshold cycle (CT) is shown at the bottom.
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