Emerging Infectious Disease ISSN: 1080-6059

Detection of SARS-associated Coronavirus in Throat Wash and Saliva in Early Diagnosis

Wei-Kung Wang*†, Shey-Ying Chen†¹, I-Jung Liu*¹, Yee-Chun Chen†, Hui-Ling Chen*, Chao-Fu Yang*, Pei-Jer Chen*, Shiou-Hwei Yeh‡, Chuan-Liang Kao*, Li-Min Huang†, Po-Ren Hsueh†, Jann-Tay Wang†, Wang-Hwei Sheng†, Chi-Tai Fang†, Chien-Ching Hung†, Szu-Min Hsieh†, Chan-Ping Su†, Wen-Chu Chiang†, Jyh-Yuan Yang§, Jih-Hui Lin§, Szu-Chia Hsieh*, Hsien-Ping Hu*, Yu-Ping Chiang*, Jin-Town Wang*, Pan-Chyr Yang†, Shan-Chwen Chang†

, and members of the SARS Research Group of the National Taiwan UniversityNational Taiwan University Hospital

Author affiliations: *National Taiwan University, Taipei, Taiwan; †National Taiwan University Hospital, Taipei, Taiwan; ‡National Health Research Institute, Taipei, Taiwan; §Center for Disease Control, Department of Health, Taipei, Taiwan; ¹S.-Y. Chen and I-J. Liu contributed equally to the work.

Main Article

Figure 1

Quantification of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) RNA by real-time reverse transcription–polymerase chain reaction (RT-PCR) assay. (A) Location of the forward and reverse primers and probe in the genome of SARS-CoV, with the genome positions shown according to the Urbani strain (20). (B) A schematic diagram of the construct, ORF1b/pCRII-TOPO, and the protocol for generating the in vitro transcribed RNA as the standard for the real-time RT-PCR assay is show

Figure 1. Quantification of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) RNA by real-time reverse transcription–polymerase chain reaction (RT-PCR) assay. (A) Location of the forward and reverse primers and probe in the genome of SARS-CoV, with the genome positions shown according to the Urbani strain (20). (B) A schematic diagram of the construct, ORF1b/pCRII-TOPO, and the protocol for generating the in vitro transcribed RNA as the standard for the real-time RT-PCR assay is shown. The relationship between known input RNA copies to the threshold cycle (CT) is shown at the bottom.

Main Article

¹S.-Y. Chen and I.-J. Liu contributed equally to the work.

Top of Page

The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.