Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

Volume 10, Number 8—August 2004

Research

Serotype III Streptococcus agalactiae from Bovine Milk and Human Neonatal Infections1

John F. Bohnsack*Comments to Author , April A. Whiting*, Gabriela Martinez†, Nicola Jones‡, Elisabeth E. Adderson§, Shauna Detrick*, Anne J. Blaschke-Bonkowsky*, Naiel Bisharat‡, and Marcelo Gottschalk†
Author affiliations: *University of Utah Health Sciences Center, Salt Lake City, Utah, USA; †Faculté de Médicine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada; ‡John Radcliffe Hospital, Oxford, United Kingdom; §St. Jude Children’s Research Hospital, Memphis, Tennessee, USA

Main Article

Table 3

Inserted sequences and sequence type (ST) of bovine strains containing the infB A allelea





Inserted sequence site



RAPD group Isolate Origin AW-10 hylB scpb-lmb ST RDP type
II
SH-96-3696
Bovine
3,400b
None
None
67
Unknownc
III
NI-96-2836
Bovine
None
IS1548
IS1548
19
III-2-like

SH-96-4807
Bovine
None
None
None
17
III-3-like

RF-96-2997
Bovine
None
None
None
94
III-1
IV-A
1003A
Human
None
IS1548
IS1548
19
III-2

AL-97-0498 SH-96-3417
Bovine
None
None
None
23
III-1

All 17 others
Bovine
None
None
No productd
23,90,92
III-1-like
IV-C
NI-96-3213
Bovine
None
None
No productd
23
III-1-like

ASS-96-666
Bovine
None
None
No productd
23
III-1-like

1007B
Human
None
IS1548
IS1548/IS1381e
86
III-2

1009A, 15888
Human
None
IS1548
IS1548
19
III-2
13228, 1009B Human None IS1548 (truncated)f IS1548 19 III-2

aRAPD, randomly amplified polymorphic DNA; RDP, restriction digest pattern.
bA 3,400-bp insert is also found in other strains from randomly amplified polymorphic DNA (RAPD) group II (see text and Table 5).
cThis strain appears to be related to the H allele strains described in Table 5.
dNo polymerase chain reaction product was produced from these strains in multiple attempts.
eIn this strain, the insertion sequence IS1381 is upstream from IS1548 in the scpb-lmb locus.
fS1548 in the hylB locus is missing 657 bp from the 3′ end of its open reading frame in this strain.

Main Article

1Presented in part at the XVth Lancefield International Symposium on Streptococci and Streptococcal Diseases, October 6–11, 2002, Goa, India.

TOP