Volume 10, Number 9—September 2004
Typing of Borrelia Relapsing Fever Group Strains
|Species||Locus||No. samples||No. variants||Aligned characters
|Base pairs||No. gapped||Polymorphisms (%)|
|Borrelia miyamotoi s.l.||IGS||33||4||474||15c||40 (8.4)||0.058|
|p66 d||19||3||617||9||38 (6.2)||0.042|
|B. lonestari||IGS||20||3||412||1||14 (3.4)||0.023|
|p66 e||7||3||346||0||5 (1.4)||0.010|
|B. hermsii||IGS||9||4||665||2||20 (3.0)||0.015|
|p66 e||5||3||516||3||8 (1.6)||0.010|
a IGS, 16S-23S rRNA gene intergenic spacer region.
b π, mean nucleotide diversity at each aligned position.
c Excludes an 81-bp indel.
d Partial p66 genes were amplified by nested PCR with outer forward and reverse primers of 5′GATTTTTCTATATTTGGACACAT and 5′AATTTAATCAGATTGTTTAGCTCTA and inner primers of 5′GACACATATCTAAAAAAGCAAACAC and 5′CTAATCCGGTTTTTACGTATATGC and following conditions: 40 cycles of 94°C for 60 s, 55°C for 120 s, and 74°C for 120 s. GenBank accession numbers for p66 genotypes are the following: B. miyamotoi s.l. type 1 (AY363722), type 2 (AY363723), type 3 (AY363724); B. lonestari type 1 (AY363689), type 2 (AY363690), type 3 (AY363691); B. hermsii type 1 (AF016408), type 2 (AF228028), and type 4 (AF116905).
e Analysis of B. lonestari and B. hermsii p66 fragments was limited to 346 of 605 bp and 516 of 608 bp, respectively, which corresponded to the shortest sequences available for all types of the individual species.
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