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Volume 10, Number 9—September 2004

Research

Detection of Anaplasma phagocytophilum DNA in Ixodes Ticks (Acari: Ixodidae) from Madeira Island and Setúbal District, Mainland Portugal

Ana Sofia Santos*Comments to Author , Maria Margarida Santos-Silva*, Victor Carlos Almeida†, Fátima Bacellar*, and John Stephen Dumler‡
Author affiliations: *Instituto Nacional de Saúde Dr. Ricardo Jorge, Águas de Moura, Portugal; †Direcção Regional de Pecuária, Funchal, Portugal; ‡The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

Main Article

Table 1

Results of PCR to detect Anaplasma phagocytophilum DNA in ticksa

Area Site Origin Ixodes ricinus
I. ventalloi
Totalc
Nymphsb Fb Mb Nymphsb Fb Mb
Madeira Island
  Paúl da Serra–Porto Moniz 1 Vegetation 6/139 0/2 0/1 142
Setúbal District Portugal Mainland
  Barris–Palmela 2 Vegetation 0/1 0/5 0/7 0/1 14
  Baixa de Palmela 3 Vegetation 0/2 0/2 0/2 1/15 0/6 0/7 34
Felis catus domesticus 0/6 1/4 10
  Picheleiros–Azeitão 4 Vegetation 0/2 0/2 0/12 0/9 0/18 43
  Azeitão 5 Vegetation 0/1 0/1 2
  Maçã–Sesimbra 6 Vegetation 0/10 0/9 0/1 0/4 0/9 33
Totalc 142 21 22 28 25 40 278

aPCR, polymerase chain reaction; F, female; M, male.
bNumber of positives ticks/number of ticks examined.
cTotal number of ticks examined.

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