Figure. Alignment of internal transcribed spacers (ITS) and partial 23S sequences with primers and probes used for real-time polymerase chain (PCR) reaction. (nucleotides [nt] 1 to 301 make up the total ITS region; nt 302 to 367 are coding for partial 23S gene). The Mycobacterium haemophilum sequence was derived from 3 different patients, but no variation was found. A, forward primer for real-time PCR; B, Mycobacterium genus–specific probe; C, M. haemophilum–specific probe; D, reverse primer for real time–polymerase chain reaction.