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Volume 11, Number 1—January 2005

Research

Mycobacterium haemophilum and Lymphadenitis in Children

Lesla E.S. Bruijnesteijn van Coppenraet*, Edward J. Kuijper*Comments to Author , Jerome A. Lindeboom†, Jan M. Prins†, and Eric C. J. Claas*
Author affiliations: *Leiden University Medical Center, Leiden, the Netherlands; †Academic Medical Centre, Amsterdam, the Netherlands

Main Article

Table 2

Results of diagnostics tests of 16 Mycobacterium haemophilum–positive patients

M. haemophilum– positive patient Acid-fast staining Culture 30°C* Genus-specific real-time PCR M. haemophilum–specific real-time PCR
1 + + +
2 +
3 + + +
4 + +
5 + +
6 + + + +
7 + + +
8 + +† + +
9 + + +‡ –‡
10 + + –‡ +‡
11 + +‡ –‡
12 + –‡ +‡
13 + + +
14 + +
15 + + +
16 + + + +
Total positive patients 9 9 13 13

*Löwenstein-Jensen (LJ) medium with added iron citrate or liquid MGIT medium with X-factor-strip added. Cultures at 30°C were performed after storage for patients 1 to 6.
†Patient material was also culture positive at 35°C.
‡Because of discrepant polymerase chain reaction (PCR) results with high threshold cycle values, the PCR was performed 5 times on these samples, which resulted in at least 2 specific positive signals for both PCRs on every sample. Therefore, the amount of mycobacterial DNA is estimated at the detection limit of the assay. The first obtained PCR result is described in the table.

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