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Volume 11, Number 10—October 2005

Dispatch

Rapid West Nile Virus Antigen Detection

Nicholas A. Panella*Comments to Author , Kristen L. Burkhalter*, Stanley A. Langevin*, Aaron C. Brault*, Lynn M. Schooley*, Brad J. Biggerstaff*, Roger S. Nasci*, and Nicholas Komar*
Author affiliations: *Centers for Disease Control and Prevention, Fort Collins, Colorado, USA

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Table 2

Log geometric mean titer (SD) of West Nile virus PFU equivalents (TaqMan RT-PCR) and PFU (plaque assay) for American Crows and House Sparrows, by swab type and day postmortem*

Species Swab type Assay Day postmortem
1 2 3 4
American Crow Oral TaqMan 4.8 (1.1) 4.7 (0.6) 4.3 (0.8) 3.5 (1.5)
Plaque 3.6 (1.9) 3.2 (1.5) 2.4 (1.6) 2.2 (2.0)
Cloacal TaqMan 5.2 (1.2) 5.5 (1.3) 4.6 (1.9) 3.5 (0.7)
Plaque 3.0 (2.4) 1.4 (1.5) 0.3 (0.7) 0.0 (0.0)
House Sparrow Oral TaqMan 4.3 (1.4) 5.0 (1.7) 4.6 (1.7) NA
Plaque 3.8 (2.0) 3.0 (1.9) 2.9 (2.5) NA
Cloacal TaqMan 3.8 (1.6) 4.3 (3.3) NA NA
Plaque 1.9 (2.9) 0.7 (0.9) NA NA

*RT-PCR, reverse transcription–polymerase chain reaction; NA, not available (valid sampling was not possible given the physical state of the bird).

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