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Volume 11, Number 10—October 2005

Research

Pyrosequencing Bacillus anthracis

Tara Wahab*, Sandra Hjalmarsson*, Ralfh Wollin*, and Lars Engstrand*Comments to Author 
Author affiliations: *Swedish Institute for Infectious Disease Control, Solna, Sweden

Main Article

Table 2

Sequence of primers used in PCR and sequencing*

Target Primer Sequence
rpoB PCR forward 5´-CCACCAACAGTAGAAAATGCCA-3´
PCR reverse b-5´-AAATTTCACCAGTTTCTGGATCTA-3´
Sequencing 911 5´-CACCAACAGTAGAAAATGC-3´
Sequencing 912 5´-TTCTTCGATCCAAAGC-3´
Sequencing 913 5´-TAAAAACAGATTGTTTA-3´
Sequencing 914 5´-AACGTTTAGCTGAAACATT-3´
pXO1 PCR forward 5´-GATATGAACCCGTACTTGTAAT-3´
PCR reverse b-5´-GAATCAGATGCATTTTTAATGG-3´
Sequencing 5´-TTTATTATGAAATAGGT-3´
pXO2 PCR forward 5´-TTCGCCTTGTGATTCCGCACT-3´
PCR reverse b-5´-GGGCGCTGGTCGTATGTTTAG-3´
Sequencing 5´- TGTTTGTCCACTTCC-3´

*PCR, polymerase chain reaction; b, biotin.

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