Volume 11, Number 10—October 2005
Lassa Fever, Nigeria, 2003 and 2004
|Patient||RT-PCR†||IgM titer‡||IgG titer‡|
|Patients with fever (n = 31)|
|Contact persons (n = 17)|
|Hospital staff (n = 12)|
*Data not shown for patients whose samples were negative in all tests. RT-PCR, reverse-transcriptase polymerase chain reaction; Ig, immunoglobulin; –, negative result.
†RT-PCR targeting the Lassa virus glycoprotein gene (4). PCR products were detected in ethidium bromide–stained gel and sequenced (GenBank accession nos. DQ010030 and DQ010031 for 04-02 and 04-10, respectively).
‡Immunofluorescence assay used cells infected with Lassa virus strain Josiah. Findings were confirmed with μ-capture and IgG enzyme-linked immunosorbent assays (data not shown).
§Lassa virus was isolated in cell culture (strain Nig04-010), and part of the L gene was sequenced (GenBank accession no. AY693637).