Figure A1. Simultaneous array detection of biological warfare agents in mixed autoclaved bacterial culture samples amplified with primer pool I (A), primer pool II (B), and wastewater samples spiked with primer pool I (C). Mixed samples were prepared by mixing Bacillus anthracis with B. thuringiensis kurstaki at 1:1, 1:5 and 1:9 ratios and Yersinia pestis with Francisella tularensis at 1:1 and 1:9 ratios. Wastewater samples were prepared by spiking individual autoclaved bacterial cultures of B. anthracis, B. thuringiensis kurstaki, Y. pestis, F. tularensis, Clostridium botulinum, and vaccinia virus at 1:10 dilution. The standard deviation (SD) of background images is 15 (n = 3), and the detection limit is 45, defined as 3 × SD.
The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.