Figure A1. Simultaneous array detection of biological warfare agents in mixed autoclaved bacterial culture samples amplified with primer pool I (A), primer pool II (B), and wastewater samples spiked with primer pool I (C). Mixed samples were prepared by mixing Bacillus anthracis with B. thuringiensis kurstaki at 1:1, 1:5 and 1:9 ratios and Yersinia pestis with Francisella tularensis at 1:1 and 1:9 ratios. Wastewater samples were prepared by spiking individual autoclaved bacterial cultures of B. anthracis, B. thuringiensis kurstaki, Y. pestis, F. tularensis, Clostridium botulinum, and vaccinia virus at 1:10 dilution. The standard deviation (SD) of background images is 15 (n = 3), and the detection limit is 45, defined as 3 × SD.