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Volume 11, Number 10—October 2005

Dispatch

Detecting Biological Warfare Agents

Linan Song*, Soohyoun Ahn*, and David R. Walt*Comments to Author 
Author affiliations: *Tufts University, Medford, Massachusetts, USA

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Table A2

Cross-reactions between 18 probes on the multiplexed array* (Download PDF [93 KB, 1 page])

Targets (100 nmol/L) Probes
Bacillus anthracis
Brucella melitensis
Bacillus thuringiensis kurstaki
Yersinia pestis
Vaccinia virus
Francisella tularensis
Clostridium botulinum
capA
pagA
SASP-B
Sequence between BMEI1658 and BMEI1659
Cry1A
plasminogen activator
Thymidine kinase
fopA
Random chromosomal DNA fragment
BA1 BA2 BA3 BA4 BA5 BA6 BM1 BM2 BTK1 BTK2 YP1 YP2 VA1 VA2 FT1 FT2 CB1 CB2
BA1 808.1 173.4 13.8 14.5 18.1 9.61 17.1 9.75 21.1 21.3 22.2 27.9 31.8 29.2 30.8 27.4 30.1 27.2
BA2 712.3 701.2 15.1 18.1 9.57 –0.28 11.9 –1.54 25.8 15.6 19.8 13.4 15.2 32.5 10.5 5.2 21.6 23.1
BA3 9.61 13.4 457.4 6.48 6.15 7.6 12.4 11.4 –2.69 13.9 5.56 6.66 11.3 24.1 8.61 –2.84 29.8 16.9
BA4 11.7 20.8 26.9 693.2 26.6 8.61 20.3 25.5 31.6 14.4 5.65 9.05 19.6 21.6 17.9 31.1 19.8 25.6
BA5 10.9 2.4 5.7 14.2 410.3 113.6 10.7 2.2 12.6 19.8 25.7 30.1 –1.6 21.7 28.2 25.8 30.1 8.78
BA6 17.7 –3.45 11.3 15.5 262.8 853.9 17.5 31.6 29.9 11.4 33.6 32.2 24.8 27.6 30.9 11.9 13.7 31.4
BM1 7.92 3.32 23.2 –3.22 18.8 11.5 723.5 –4.28 32.2 –4.99 20.8 25.8 18.2 17.3 29.3 27.9 25.9 14.6
BM2 3.08 3.84 26.8 6.78 3.96 5.67 24.1 973.3 21.3 1.11 15.9 17.6 19.9 32.8 13.9 24.4 34.4 12.9
BTK1 14.1 6.1 30.4 6.35 15.6 9.77 17.1 6.05 1423.1 232.8 32.4 19.2 19.7 12.5 16.6 26.3 8.95 23.1
BTK2 27.7 –0.91 3.39 6.8 8.39 4.56 10.3 9.59 425.8 1288.6 22.1 8.16 9.8 18.9 28.2 19 7.84 19.4
YP1 12.5 –0.79 28 4.56 12.5 5.11 8.88 20.6 12.9 32.1 2089 588.7 23.6 24.6 179.7 25.7 15.9 29.2
YP2 23.4 32.1 23.6 14.2 –1.58 29.3 15.4 19.1 28.1 23.8 1491.5 1784 14.5 11.3 9.7 7.05 23.9 14.6
VA1 8.9 24.9 25.3 4.71 8.61 18.7 13.6 8.61 3.12 9.87 12.7 16.6 1536.9 1.75 25.4 23.8 17.7 11.8
VA2 43.1 15.9 4.59 8.94 7.18 23.2 29.4 3.25 15.4 14.7 26.7 12 18.3 1703.4 23.9 –0.76 26.3 29.7
FT1 19.8 19.7 8.77 25.6 14.6 4.65 32.1 14.7 6.96 1.56 14.9 0.65 0.56 0.78 793 26.8 28.5 20.5
FT2 25.4 8.4 11.5 16.9 19.7 7.98 9.94 17.9 12.5 25.1 4.44 14.5 8.3 13.4 216.7 456.9 8.99 27.9
CB1 23.1 5.68 16.9 20.1 27.2 15.3 14.1 9.11 7.46 16.1 4.6 19 28.3 171.6 4.93 24.8 843.1 18.5
CB2 14.8 11.2 8.45 8.16 5.45 24.6 20.9 3.25 1.62 21.9 25.8 23.5 13.3 18.0 28.2 21.2 89.1 592.1

*The numbers are net fluorescence intensities (in arbitrary units) obtained by subtracting the average intensity value of the background from that of the hybridization signal. The specific response for each probe is indicated in red. The cross responses between probes designed for the same biological warfare agent (BWA) are indicated in pink, and the cross-reactions between probes from different BWAs are marked with blue. The standard deviation (SD) of background is 15 (n = 3), and the detection limit of array hybridization for each microsphere probe is 45, defined as 3 × SD.

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