Porcine Noroviruses Related to Human Noroviruses
Figure 5. Antigenic cross-reactivity between human genogroup (G) II norovirus (NoV) capsid proteins and a pig convalescent-phase antiserum (LL616) against porcine QW101-like (GII-18) NoV was determined by Western blot. The CsCl-gradient purified viruslike particles (1,250 ng) were separated by sodium dodecyl sulfate 10% polyacrylamide gel electrophoresis, blotted onto nitrocellulose membranes, and tested with LL616. The sucrose-cushion (40%, wt/vol) purified Sf9 insect cell proteins acted as a negative control (lane 8). Lane 1, molecular weight marker (kDa); lanes 2–7, Hu/GI-3/Desert Shield, Hu/GII-1/Hawaii, Hu/GII-3/Toronto, Hu/GII-4/MD145, Hu/GII-4/HS66, and Hu/GII-6/Florida, respectively.