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Volume 11, Number 12—December 2005

Research

Postepizootic Persistence of Venezuelan Equine Encephalitis Virus, Venezuela

Juan-Carlos Navarro*, Gladys Medina†, Clovis Vasquez‡, Lark L. Coffey§, Eryu Wang§, Alexander Suárez*‡, Hernán Biord#, Marlene Salas¶, and Scott C. Weaver§Comments to Author 
Author affiliations: *Universidad Central de Venezuela, Caracas, Venezuela; †Instituto Nacional de Investigaciones Agropecuarias, Maracay, Venezuela; ‡Instituto Nacional de Higiene, Caracas, Venezuela; #Sociedad Venezolana de Ciencias Naturales, Venezuela; ¶Ministerio de Agricultura y Tierras, Barinas, Venezuela; §University of Texas Medical Branch, Galveston, Texas, USA

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Table 3

Results of the blocking ELISA to determine VEEV subtypes producing seroconversion in HI- and PRNT-positive bovines from Arismendi municipality, Barinas State, Venezuela*

Sample no.† % inhibition of epizootic–specific MAb 1A3A–5 binding at indicated serum dilution
% inhibition of enzootic–specific MAb 1A1B–9 binding at indicated serum dilution
1:4 1:12 1:36 1:4 1:12 1:36
156 3.0 8.5 –5.0 52.4 17.8 –34.3
158 12.4 13.9 –3.6 27.0 –20.4 –37.0
166 35.6 12.5 6.2 30.7 42.7 –21.0
167 59.6 42.0 3.7 65.7 17.1 –9.9
172 –11.7 –15.4 –13.8 37.5 32.1 –19.0
175 8.2 5.0 2.1 48.5 21.5 –20.0
178 23.1 15.1 2.2 69.7 44.9 –5.9
204 –1.1 –10.8 –22.4 75.8 56.4 29.7
Negative control† 4.1 6.6 5.4 15.2 23.1 –5.7
Negative control† 9.4 14.1 12.0 8.0 14.5 15.6
Negative control† 5.8 11.4 11.3 17.5 23.2 11.0
Positive control† 18.8 9.5 –8.0 65.3 51.0 29.0
Positive control† 16.4 1.4 1.7 50.1 16.7 18.9

*ELISA, enzyme-linked immunosorbent assay; VEEV, Venezuelan equine encephalitis virus; HI, hemagglutination-inhibition; PRNT, plaque reduction neutralization test; MAb, monoclonal antibody.

†Negative controls are bovine sera from locations with no history of VEE; positive controls are human sera from virus isolation–confirmed, subtype ID VEEV infections (note that the assay is species–independent).

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