Volume 11, Number 12—December 2005
Research
Postepizootic Persistence of Venezuelan Equine Encephalitis Virus, Venezuela
Juan-Carlos Navarro*, Gladys Medina†, Clovis Vasquez‡, Lark L. Coffey§, Eryu Wang§, Alexander Suárez*‡, Hernán Biord#, Marlene Salas¶, and Scott C. Weaver§
Table 3
Results of the blocking ELISA to determine VEEV subtypes producing seroconversion in HI- and PRNT-positive bovines from Arismendi municipality, Barinas State, Venezuela*
| Sample no.† | % inhibition of epizootic–specific MAb 1A3A–5 binding at indicated serum dilution |
% inhibition of enzootic–specific MAb 1A1B–9 binding at indicated serum dilution |
||||
|---|---|---|---|---|---|---|
| 1:4 | 1:12 | 1:36 | 1:4 | 1:12 | 1:36 | |
| 156 | 3.0 | 8.5 | –5.0 | 52.4 | 17.8 | –34.3 |
| 158 | 12.4 | 13.9 | –3.6 | 27.0 | –20.4 | –37.0 |
| 166 | 35.6 | 12.5 | 6.2 | 30.7 | 42.7 | –21.0 |
| 167 | 59.6 | 42.0 | 3.7 | 65.7 | 17.1 | –9.9 |
| 172 | –11.7 | –15.4 | –13.8 | 37.5 | 32.1 | –19.0 |
| 175 | 8.2 | 5.0 | 2.1 | 48.5 | 21.5 | –20.0 |
| 178 | 23.1 | 15.1 | 2.2 | 69.7 | 44.9 | –5.9 |
| 204 | –1.1 | –10.8 | –22.4 | 75.8 | 56.4 | 29.7 |
| Negative control† | 4.1 | 6.6 | 5.4 | 15.2 | 23.1 | –5.7 |
| Negative control† | 9.4 | 14.1 | 12.0 | 8.0 | 14.5 | 15.6 |
| Negative control† | 5.8 | 11.4 | 11.3 | 17.5 | 23.2 | 11.0 |
| Positive control† | 18.8 | 9.5 | –8.0 | 65.3 | 51.0 | 29.0 |
| Positive control† | 16.4 | 1.4 | 1.7 | 50.1 | 16.7 | 18.9 |
*ELISA, enzyme-linked immunosorbent assay; VEEV, Venezuelan equine encephalitis virus; HI, hemagglutination-inhibition; PRNT, plaque reduction neutralization test; MAb, monoclonal antibody.
†Negative controls are bovine sera from locations with no history of VEE; positive controls are human sera from virus isolation–confirmed, subtype ID VEEV infections (note that the assay is species–independent).
