Figure 2. . Detection of 3 human coronavirus (CoV) in a mixture. The deconvoluted (neutral mass) mass spectra obtained for the RNA-dependent RNA polymerase primer for the 3 human CoV, HCoV-229E, HCoV-OC43, and severe acute respiratory syndrome–associated CoV, which were tested individually and in a mixture are shown. Forward and reverse amplicons are shown with the measured monoisotopic masses for each strand. Colors of the monoisotopic masses for the mixed spectra correspond to the individual viral species.