Longitudinally Profiling Neutralizing Antibody Response to SARS Coronavirus with Pseudotypes
Nigel J. Temperton* , Paul K. Chan†, Graham Simmons‡, Maria C. Zambon§, Richard S. Tedder*, Yasuhiro Takeuchi*, and Robin A. Weiss*
Author affiliations: *University College London, London, United Kingdom; †Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, People’s Republic of China; ‡University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA; §Health Protection Agency Central Public Health Laboratory, London, United Kingdom
Figure 1. . Infectivity of retroviral severe acute respiratory syndrome–associated coronavirus (SARS-CoV) spike protein (S) pseudotypes on target cells. SARS-CoV S-mediated infection of human 293T, TE671, and Quail QT6/ACE2 was assessed. Murine leukemia virus (MLV) or HIV pseudotypes bearing either the pantropic vesicular stomatitis virus envelope protein (VSV-G) as a positive control, or the SARS-CoV S, were added to target cells. After 72 h, green fluorescent protein (GFP)–positive cells were counted by fluorescence-activated cell sorter analysis. Infection titers are given as infectious units per milliliter (IU/mL). Arrow indicates that infection titer was less than the detection limit, 102 IU/mL.
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