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Volume 11, Number 3—March 2005

Research

Longitudinally Profiling Neutralizing Antibody Response to SARS Coronavirus with Pseudotypes

Nigel J. Temperton*Comments to Author , Paul K. Chan†, Graham Simmons‡, Maria C. Zambon§, Richard S. Tedder*, Yasuhiro Takeuchi*, and Robin A. Weiss*
Author affiliations: *University College London, London, United Kingdom; †Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, People’s Republic of China; ‡University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA; §Health Protection Agency Central Public Health Laboratory, London, United Kingdom

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Figure 3

Severe acute respiratory syndrome–associated coronavirus (SARS-CoV) neutralizing antibody–positive rate by time of blood sample collection (days after onset of fever). Black bars represent the number of patients tested for neutralizing antibodies (Nab). White bars represent the number of patients whose assayed samples were positive for neutralizing antibodies (Nab+). Samples are considered positive for Nab if the 90% neutralizing antibody titer determined by using murine leukemia virus (MLV) (SA

Figure 3. . Severe acute respiratory syndrome–associated coronavirus (SARS-CoV) neutralizing antibody–positive rate by time of blood sample collection (days after onset of fever). Black bars represent the number of patients tested for neutralizing antibodies (Nab). White bars represent the number of patients whose assayed samples were positive for neutralizing antibodies (Nab+). Samples are considered positive for Nab if the 90% neutralizing antibody titer determined by using murine leukemia virus (MLV) (SARS) pseudotypes is >10. Line plot with open white circles shows the geometric mean (GM) Nab titer within each time frame. IC90, 90% inhibitory concentration.

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