Volume 11, Number 5—May 2005
Dispatch
Highly Pathogenic H5N1 Influenza Virus in Smuggled Thai Eagles, Belgium
Steven Van Borm*
, Isabelle Thomas†, Germaine Hanquet†, Bénédicte Lambrecht*, Marc Boschmans*, Gérald Dupont†, Mireille Decaestecker*, René Snacken†, and Thierry van den Berg*
, Isabelle Thomas†, Germaine Hanquet†, Bénédicte Lambrecht*, Marc Boschmans*, Gérald Dupont†, Mireille Decaestecker*, René Snacken†, and Thierry van den Berg*Table
Primers used for human (h) and avian (a) diagnosis, subtyping, and sequencing
| Primer | Specificity | Host | Primer sequence (5´–3´) | PCR product (bp) | Reference* |
|---|---|---|---|---|---|
| NP1200F | Flu A | h, a | CAGRTACTGGGCHATAAGRAC | 330 | (6) |
| NP1529R | Flu A | h, a | GCATTGTCTCCGAAGAAATAAG | (6) | |
| NP 1222 F | Flu A | h | CAGRAGYGGAGGRAAYACYAAYC | 245 | This study (IT) |
| NP 1467 R | Flu A | h | CGGGYTCGYTGCCTTYTCGTC | This study (IT) | |
| BHAA | Flu B | h | GTGACTGGTGTGATACCACT | 900 | (7) |
| BHAD2 | Flu B | h | TGTTTTCACCATATTGGGGC | (7) | |
| BHAB | Flu B | h | CATTTTGCAAATCTCAAAGG | 767 | (7) |
| BHAC2 | Flu B | h | TGGAGGCAATCTGCTTCACC | (7) | |
| H5-155F | Flu A/H5 | h, a | ACACATGCYCARGACATACT | 545 | (6) |
| H5-699R | Flu A/H5 | h, a | CTYTGRTTYAGTGTTGATGT | (6) | |
| H5 1 F | Flu A/H5 | h | GCCATTCCACAACATACACCC | 401 | Mod. from (8) |
| H5 2 R | Flu A/H5 | h | TTAATTCTCTATCCTCCTTTCCAA | Mod. from (8) | |
| H5 IS 1F | Flu A/H5 | h | CTTGCGACTGGGCTCAGAAAT | 645 | This study (IT) |
| H5 IS 2R | Flu A/H5 | h | CCTTCCAACGGCCTCAAACTG | This study (IT) | |
| β act 5 | βactine | h | AACACCCCAGCCATGTAC | 181 | This study (IT) |
| β act 6 | βactine | h | GTAGTCAGTCAGGTCCCG | This study (IT) | |
| β act 7 | βactine | h | AACACCCCAGCCATGTAC | 144 | This study (IT) |
| β act 8 | βactine | h | GCCAGCCAGGTCCAGACG | This study (IT) | |
| 18S-F908 | 18S | a | AGCGAAAGCATTTGCCAAGA | 401 | This study (SVB) |
| 18S-R1309 | 18S | a | AGTCTCGTTCGTTATCGGAATT | This study (SVB) | |
| H5-614F | HA sequencing | a | GARGAYCTTYTRRTAHTRTGG | 645 | This study (MB) |
| H5-1259R | HA sequencing | a | CYTCRAAHTGRGTGTTCATT | This study (MB) |
*IT, Isabelle Thomas; SVB, Steven Van Borm; MB, Marc Boschmans; Mod., modeled.
