Community-acquired Methicillin-resistant Staphylococcus aureus, Uruguay
Figure 2. . . Dendrogram of pulsed-field gel electrophoresis (PFGE) banding pattern of representative Uruguay clone. Pulsotypes of representative Uruguay strains, a CA-MRSA strain isolated in the United States (MW2), 3 CA-MRSA strains isolated in Australia (A803355, A823549, and E802537), and a Japanese strain isolated from an outpatient (81/108) were compared by using a BioNumerics software program (Applied Maths, Sint-Martens-Latem, Belgium). Similarity coefficient was calculated by using Pearson correlation with position tolerance of 5%, and cluster analysis was performed by the unweighted pair-group method. Pulsotypes in parentheses indicate the types previously reported (7). PFGE was performed for 22 h with a CHEF MAPPER (Bio-Rad, Hercules, CA, USA) with a pulse time of 5 s to 40 s. SmaI-restriction patterns of the tested strains and reference strains were compared by using BioNumerics software. Genotypes of representative strains were determined by multilocus sequence typing as described by Enright et al. (9). Sequence type (ST) and clonal complex were assigned using programs in the S. aureus multilocus sequence typing database (http://www.mlst.net).