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Volume 11, Number 6—June 2005

Research

Community-associated Methicillin-resistant Staphylococcus aureus, Canada

Michael R. Mulvey*Comments to Author , Laura MacDougall†‡, Brenda Cholin§, Greg Horsman¶, Melanie Fidyk#, Shirley Woods**, and the Saskatchewan CA-MRSA Study Group
Author affiliations: *National Microbiology Laboratory, Winnipeg, Manitoba, Canada; †British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada; ‡Health Canada, Ottawa, Ontario, Canada; §Prairie North Health Region, North Battleford, Saskatchewan, Canada; ¶Saskatchewan Health Provincial Laboratory, Regina, Saskatchewan, Canada; #Kelsey Trail Health Region, Nipawin, Saskatchewan, Canada; **Northern Intertribal Health Authority, Prince Albert, Saskatchewan, Canada

Main Article

Table 1

DNA oligonucleotides used in this study

Primer/Probe Oligonucleotide sequence Final concentration (μmol)
mecA forward 5´ GGC AAT ATT ACC GCA CCT CA 3´ 0.30
mecA reverse 5´ GTC TGC CAC TTT CTC CTT GT 3´ 0.30
mecA probe 5´ FAM - AGA TCT TAT GCA AAC TTA ATT GGC AAA TCC - TAMRA 3´ 0.10
nuc forward 5´ CAA AGC ATC AAA AAG GTG TAG AGA 3´ 0.05
nuc reverse 5´ TTC AAT TTT CTT TGC ATT TTC TAC CA 3´ 0.05
nuc probe 5´ VIC - TTT TCG TAA ATG CAC TTG CTT CAG GAC CA - TAMRA 3´ 0.05

Main Article

1Members of the Saskatchewan CA-MRSA Study Group: N. Antonishyn, Provincial Laboratory Saskatchewan Health, Regina, SK; T. Du, National Microbiology Laboratory, Winnipeg, MB; J. Embil, University of Manitoba, Winnipeg, MB; A. Graessli, University of Manitoba, Winnipeg, MB; J. Irvine, Keewatin Yatthe & Mamaweetan Churchill Regional Health Authority, La Ronge, SK; M. Khan, Kelsey Trail Health Region, Melfort, SK; S. Martin, Kelsey Trail Health Region, Nipawin, SK; R. McDonald, Provincial Laboratory Saskatchewan Health, Regina, SK; M. Nsungu, Northern Intertribal Health Authority, Prince Albert, SK; S. Paton, Public Health Agency of Canada, Ottawa, ON; C. Celin, National Microbiology Laboratory, Winnipeg, MB; D. Spreitzer, National Microbiology Laboratory, Winnipeg, MB; D. Stockdale, Keewatin Yatthe & Mamaweetan Churchill Regional Health Authority, La Ronge, SK.

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