Volume 11, Number 6—June 2005
Dispatch
Rifampicin Resistance in Tuberculosis Outbreak, London, England
Claire Jenkins*
, Alleyna P. Claxton†, Robert J. Shorten*, Timothy D. McHugh*, and Stephen H. Gillespie*
, Alleyna P. Claxton†, Robert J. Shorten*, Timothy D. McHugh*, and Stephen H. Gillespie*
Table
Sequences of 6 sets of primers designed to amplify 6 overlapping fragments of the rpoB gene from Mycobacterium tuberculosis isolates
| Fragment | Amplicon size | Primer position | Primers |
|---|---|---|---|
| 1 | 697 bp | –135 to –112* 541–562 | Forward 5´ GTT TAG TTG CGT GCG TGC Reverse 5´ CTTGTCAAT GGT CTC GTC GAA |
| 2 | 688 bp | 451–472 1119–1139 | Forward 5´ TTC CCG ATG ATG ACC GAG AAG Reverse 5´ GGA TCA GCT CGC CGA CCG TA |
| 3 | 706 bp | 1029–1048 1714–1735 | Forward 5´ CGA GGG TCA GAC CAC GAT G Reverse 5´ GCG GGG CGA GAC GTC CAT GTA |
| 4 | 681 bp | 1624–1645 2284–2305 | Forward 5´ ATC GAT GCG GAC GGT CGC TTC Reverse 5´ CGG GAT GTC GCG GGT GAT CTC |
| 5 | 811 bp | 2194–2215 2887–3005 | Forward 5´ TCC AAC CGC CTG GTC GAA GAG Reverse 5´ CGT CGA CAC AAT GGC GTT |
| 6 | 847 bp | 2797–2815 +113 to +135* | Forward 5´ TGT GCC CAC AGC GGC TGG Reverse 5´ CTT TTT GAC CTC GCC ATA GGA C |
*Denotes a primer position in the sequence before the start (–) or after the end (+) of the rpoB gene sequence.
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