Skip directly to search Skip directly to A to Z list Skip directly to site content
CDC Home

Volume 11, Number 6—June 2005

Dispatch

Rifampicin Resistance in Tuberculosis Outbreak, London, England

Claire Jenkins*Comments to Author , Alleyna P. Claxton†, Robert J. Shorten*, Timothy D. McHugh*, and Stephen H. Gillespie*
Author affiliations: *Royal Free Hospital, London, United Kingdom; †Homerton Hospital, London, United Kingdom

Main Article

Table

Sequences of 6 sets of primers designed to amplify 6 overlapping fragments of the rpoB gene from Mycobacterium tuberculosis isolates

Fragment Amplicon size Primer position Primers
1 697 bp –135 to –112*
541–562 Forward 5´ GTT TAG TTG CGT GCG TGC
Reverse 5´ CTTGTCAAT GGT CTC GTC GAA
2 688 bp 451–472
1119–1139 Forward 5´ TTC CCG ATG ATG ACC GAG AAG
Reverse 5´ GGA TCA GCT CGC CGA CCG TA
3 706 bp 1029–1048
1714–1735 Forward 5´ CGA GGG TCA GAC CAC GAT G
Reverse 5´ GCG GGG CGA GAC GTC CAT GTA
4 681 bp 1624–1645
2284–2305 Forward 5´ ATC GAT GCG GAC GGT CGC TTC
Reverse 5´ CGG GAT GTC GCG GGT GAT CTC
5 811 bp 2194–2215
2887–3005 Forward 5´ TCC AAC CGC CTG GTC GAA GAG
Reverse 5´ CGT CGA CAC AAT GGC GTT
6 847 bp 2797–2815
+113 to +135* Forward 5´ TGT GCC CAC AGC GGC TGG
Reverse 5´ CTT TTT GAC CTC GCC ATA GGA C

*Denotes a primer position in the sequence before the start (–) or after the end (+) of the rpoB gene sequence.

Main Article

Top of Page

USA.gov: The U.S. Government's Official Web PortalDepartment of Health and Human Services
Centers for Disease Control and Prevention   1600 Clifton Rd. Atlanta, GA 30333, USA
800-CDC-INFO (800-232-4636) TTY: (888) 232-6348 - Contact CDC–INFO