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Volume 11, Number 7—July 2005

Research

Influenza A H5N1 Replication Sites in Humans

Mongkol Uiprasertkul*, Pilaipan Puthavathana*, Kantima Sangsiriwut*, Phisanu Pooruk*, Kanittar Srisook*, Malik Peiris†, John M. Nicholls†, Kulkanya Chokephaibulkit*, Nirun Vanprapar*, and Prasert Auewarakul*Comments to Author 
Author affiliations: *Mahidol University, Bangkok, Thailand; †University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China

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Figure 2

A) Detection of H5 influenza viral RNA in lungs, intestines, and spleen by reverse transcription-polymerase chain reaction (RT-PCR). B) Strand-specific RT-PCR detected positive-stranded viral RNA only in lungs and intestines but not in spleen. +/–, total RNA; –, negative-stranded RNA; +, positive-stranded RNA. RT-PCR products of an infected cell culture pellet and supernatant are shown as a control for proper amplification of the specific strands (lower panel). C) Tumor necrosis factor-α (TNF-α)

Figure 2. . A) Detection of H5 influenza viral RNA in lungs, intestines, and spleen by reverse transcription-polymerase chain reaction (RT-PCR). B) Strand-specific RT-PCR detected positive-stranded viral RNA only in lungs and intestines but not in spleen. +/–, total RNA; –, negative-stranded RNA; +, positive-stranded RNA. RT-PCR products of an infected cell culture pellet and supernatant are shown as a control for proper amplification of the specific strands (lower panel). C) Tumor necrosis factor-α (TNF-α) mRNA was detected by RT-PCR only in lung tissue of the patient but not in lung tissue from a healthy control.

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