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Volume 11, Number 7—July 2005

Research

Influenza A H5N1 Replication Sites in Humans

Mongkol Uiprasertkul*, Pilaipan Puthavathana*, Kantima Sangsiriwut*, Phisanu Pooruk*, Kanittar Srisook*, Malik Peiris†, John M. Nicholls†, Kulkanya Chokephaibulkit*, Nirun Vanprapar*, and Prasert Auewarakul*Comments to Author 
Author affiliations: *Mahidol University, Bangkok, Thailand; †University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China

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Figure 3

Immunohistochemical analysis showing influenza A antigen-specific staining in nuclei of cells lining the alveoli (A). To identify the cell type, slides from consecutive sections were stained with anti-influenza A antibody (B) and double-stained with antiinfluenza A and antisurfactant antibodies (C). The sections were mapped, and the same area in each section was examined. Viral antigen-positive cells were stained both intranuclearly with antiinfluenza antibody and intracytoplasmically with antis

Figure 3. . Immunohistochemical analysis showing influenza A antigen-specific staining in nuclei of cells lining the alveoli (A). To identify the cell type, slides from consecutive sections were stained with anti-influenza A antibody (B) and double-stained with antiinfluenza A and antisurfactant antibodies (C). The sections were mapped, and the same area in each section was examined. Viral antigen-positive cells were stained both intranuclearly with antiinfluenza antibody and intracytoplasmically with antisurfactant antibody, indicating that the viral antigen-positive cells were type II pneumocytes. Viral antigen-positive cell are marked by circles (magnification x400).

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