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Volume 11, Number 8—August 2005

Research

Spoligotyping and Mycobacterium tuberculosis

Andrea Gori*Comments to Author , Alessandra Bandera*, Giulia Marchetti*, Anna Degli Esposti*, Lidia Catozzi*, Gian Piero Nardi*, Lidia Gazzola*, Giulio Ferrario*, Jan D.A. van Embden†, Dick van Soolingen†, Mauro Moroni*, and Fabio Franzetti*
Author affiliations: *University of Milan, Milan, Italy; †National Institute of Public Health and Environmental Protection, Bilthoven, the Netherlands

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Table 2

Comparison between spoligotyping and culture results in 350 acid-fast bacilli–positive samples

Mycobacteria grown in culture No. episodes Results of spoligotyping from clinical samples
Results of spoligotyping from liquid medium
Results of spoligotyping from solid medium
Total
Positive Negative Positive Negative Positive Negative Positive Negative
M. tuberculosis 77 54 1 53 5 75 0 182 6
M. avium 28 0 15 0 20 2 31 2 66
M. gordonae 15 0 0 0 12 0 14 0 26
M. xenopi 8 0 2 1 5 1 6 2 13
M. kansasii 2 0 1 0 0 0 2 0 3
M. chelonae 2* 0 2 0 2 0 2 0 6
Other 6† 1 1 0 4 1 4 2 9
No growth 26 9 21 1 2 0 0 10 23
Subtotal 64 43 55 50 79 59 198 152
Total 164‡ 107 105 138 350
Sensitivity, %§ 98.2 (71.1–98.4) 91.4 (75.7–90) 100 96.8 (86.2–97)
Specificity, %§ 95.5 (67.7–97.7) 97.7 (95.6–97.8) 93.6 95.3 (88.4–96.1)

*Automated DNA sequencing rather than culture growth of M. chelonae confirmed one of these cases.
†Other, 1 M. fortuitum, 1 M. asiaticum, and 4 nontyped mycobacteria.
‡16 patients had ≥2 episodes of mycobacterial infections.
§Sensitivity and specificity were calculated without including the culture-negative specimens. Values in parentheses are ranges that include specimens with no growth.

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