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Volume 11, Number 8—August 2005

Dispatch

Influenza A H5N1 Detection

Enders K.O. Ng*, Peter K.C. Cheng*, Antia Y.Y. Ng*, T.L. Hoang†, and Wilina W.L. Lim*Comments to Author 
Author affiliations: *Department of Health, Hong Kong Special Administrative Region, China; †National Institute of Hygiene and Epidemiology, Hanoi, Vietnam

Main Article

Table 2

Detection sensitivity of polymerase chain reaction and rapid antigen test for H5N1*

Test
Dilution†
103
104
105
106
107
108
Rapid antigen test‡ + +
Conventional nested RT-PCR + + + +
Real-time RT-PCR + + + + +

*RT-PCR, reverse transcription–polymerase chain reaction.
†Serial 10-fold dilution with a concentration of 108 50% tissue culture infective doses/mL of rhabdomyosarcoma cell culture fluid.
‡Binax NOW; +, positive; –, negative.

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