TY - JOUR AU - Glazunova, Olga AU - Roux, VĂ©ronique AU - Freylikman, Olga AU - Sekeyova, Zuzana AU - Fournous, Ghislain AU - Tyczka, Judith AU - Tokarevich, Nikolai AU - Kovacova, Elena AU - Marrie, Thomas AU - Raoult, Didier T1 - Coxiella burnetii Genotyping T2 - Emerging Infectious Disease journal PY - 2005 VL - 11 IS - 8 SP - 1211 SN - 1080-6059 AB - Coxiella burnetii is a strict intracellular bacterium with potential as a bioterrorism agent. To characterize different isolates of C. burnetii at the molecular level, we performed multispacer sequence typing (MST). MST is based on intergenic region sequencing. These regions are potentially variable since they are subject to lower selection pressure than the adjacent genes. We screened 68 spacers in 14 isolates and selected the 10 that exhibited the most variation. These spacers were then tested in 159 additional isolates obtained from different geographic areas or different hosts or were implicated in different manifestations of human disease caused by C. burnetii. The sequence analysis yielded 30 different allelic combinations. Phylogenic analysis showed 3 major clusters. MST allows easy comparison and exchange of results obtained in different laboratories and could be a useful tool for identifying bacterial strains. KW - Keywords: Coxiella burnetii KW - Q fever KW - Phylogeny KW - Bacterial typing KW - DNA sequence analysis DO - 10.3201/eid1108.041354 UR - https://wwwnc.cdc.gov/eid/article/11/8/04-1354_article ER - End of Reference