Figure A1. . Representative results of groEL heminested polymerase chain reaction (PCR) and HindIII digestion pattern. Lane 1: result of the first PCR round obtained with the strain NCH-1 used as control. Lanes 2, 4: results of the heminested PCR obtained with NCH-1 and with a symptomatic dog, respectively. Lanes 3, 5: HindIII digestions of amplicons shown in lanes 2 and 4, respectively. M:100 bp ladder.