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Volume 11, Number 8—August 2005

Letter

Anaplasma phagocytophilum, Sardinia, Italy

Alberto Alberti*Comments to Author , Maria Filippa Addis*, Olivier Sparagano†, Rosanna Zobba*, Bernardo Chessa*, Tiziana Cubeddu*, Maria Luisa Pinna Parpaglia*, Mauro Ardu*, and Marco Pittau*
Author affiliations: *Università degli Studi di Sassari, Sassari, Italy; †University of Newcastle, Newcastle upon Tyne, United Kingdom

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Figure A1

Representative results of groEL heminested polymerase chain reaction (PCR) and HindIII digestion pattern. Lane 1: result of the first PCR round obtained with the strain NCH-1 used as control. Lanes 2, 4: results of the heminested PCR obtained with NCH-1 and with a symptomatic dog, respectively. Lanes 3, 5: HindIII digestions of amplicons shown in lanes 2 and 4, respectively. M:100 bp ladder.

Figure A1. . Representative results of groEL heminested polymerase chain reaction (PCR) and HindIII digestion pattern. Lane 1: result of the first PCR round obtained with the strain NCH-1 used as control. Lanes 2, 4: results of the heminested PCR obtained with NCH-1 and with a symptomatic dog, respectively. Lanes 3, 5: HindIII digestions of amplicons shown in lanes 2 and 4, respectively. M:100 bp ladder.

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