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Volume 11, Number 8—August 2005

Letter

Neisseria meningitidis Endotoxin and Capsule Transmission by Transplantation

Nareg Roubinian*, Beth D. Kirkpatrick*, Freyja Lynn†, Jonathan Zenilman‡, and Margaret Bash†Comments to Author 
Author affiliations: *University of Vermont College of Medicine, Burlington, Vermont, USA; †US Food and Drug Administration, Rockville, Maryland, USA; ‡Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

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Figure

Pre- and posttransplantation serum antibodies as measured by enzyme-linked immunosorbent assay (ELISA). A) Immunoglobulin (Ig) G antibodies to Neisseria meningitidis serogroup C capsular polysaccharide (CPS) determined as described by Arakere and Frasch (7) with minor modifications. Samples were run in duplicate at 8 serial dilutions, and antibody concentrations were calculated relative to the standard reference serum lot CDC 1992 (courtesy of G. Carlone, Centers for Disease Control and Preventi

Figure. . Pre- and posttransplantation serum antibodies as measured by enzyme-linked immunosorbent assay (ELISA). A) Immunoglobulin (Ig) G antibodies to Neisseria meningitidis serogroup C capsular polysaccharide (CPS) determined as described by Arakere and Frasch (7) with minor modifications. Samples were run in duplicate at 8 serial dilutions, and antibody concentrations were calculated relative to the standard reference serum lot CDC 1992 (courtesy of G. Carlone, Centers for Disease Control and Prevention, Atlanta, GA). B) IgM antibodies to N. meningitidis lipooligosaccharide (LOS) immunotypes (L2, L3, L7, L9). ELISA to detect antibodies to LOS immunotypes L2, L3, L7, or L9 was performed as described (8), with minor modifications, by using goat antihuman IgG (γ-specific, Kirkegaard & Perry, Gaithersburg, MD, USA) or goat antihuman IgM (μ-specific, Sigma, St. Louis, MO, USA) conjugated to alkaline phosphatase. Samples were run in duplicate at 4 serial dilutions. OD, optical density.

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