Figure 1. . Ethidium bromide stain of 2% agarose gel showing reverse transcription–polymerase chain reaction (RT-PCR) products of human coronaviruses (HCoVs). A) Simple 1-step RT-PCR (HCoV-NL63, gene N): lane 1, size markers (100 bp); lane 2, negative control RT-PCR mix; lane 3, positive control HCoV-NL63. B) Multiplex 1-step RT-PCR (HCoVs NL63, OC43, 229E): lane 4, size markers (100 bp); lane 5, negative control RT-PCR mix; lane 6, positive control HCoV-NL63; lane 7, positive control HCoV-OC43; lane 8, positive control HCoV-229E; lane 9, mix of 3 positive control HCoVs (NL63, OC43, and 229E).
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