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Volume 12, Number 1—January 2006

Research

SARS–associated Coronavirus Replication in Cell Lines

Matthew Kaye*Comments to Author , Julian Druce*, Thomas Tran*, Renata Kostecki*, Doris Chibo*, Jessica Morris*, Mike Catton*, and Chris Birch*
Author affiliations: *Victorian Infectious Diseases Reference Laboratory, North Melbourne, Victoria, Australia

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Figure 2

Cycle threshold (Ct) changes measured by real-time polymerase chain reaction versus days after infection of the indicated cell lines. The cell-free sample had an initial Ct of 31, which rose to 40 by day 11. Reductions in the Ct or flat-line Ct values (e.g., COS cells) indicate replication of the virus. Continued increases in Ct above the initial value of 31 by days 7 and 11 indicate failure to replicate.

Figure 2. Cycle threshold (Ct) changes measured by real-time polymerase chain reaction versus days after infection of the indicated cell lines. The cell-free sample had an initial Ct of 31, which rose to 40 by day 11. Reductions in the Ct or flat-line Ct values (e.g., COS cells) indicate replication of the virus. Continued increases in Ct above the initial value of 31 by days 7 and 11 indicate failure to replicate.

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