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Volume 12, Number 10—October 2006

Letter

Enterovirus 75 and Aseptic Meningitis, Spain, 2005

Ana Avellón*Comments to Author , Gurutze Rubio†, Gustavo Palacios‡, Inmaculada Casas*, Nuria Rabella§, Gabriel Reina¶, Cármen Pérez#, W. Ian Lipkin‡, and Gloria Trallero*
Author affiliations: *Carlos III Institute of Health, Majadahonda, Spain; †Cruzes Hospital, Bilbao, Spain; ‡Columbia University, New York, New York, USA; §Sant Pau y Santa Creu Hospital, Barcelona, Spain; ¶Virgen de las Nieves Hospital, Granada, Spain; #Doctor Negrín Hospital, Las Palmas de Gran Canaria, Spain

Main Article

Figure

Phylogenetic analysis of complete VP1 sequences of Spanish enterovirus (EV) isolates (GenBank accession nos. DQ468137–DQ468142), the new proposed EV75 sequences (AY556063–AY556070 and AY919545), and prototype EV sequences (echovirus [E] 5, AJ241425; E31, AJ241435; E2, AF081315; E15, AJ241429; E14, AJ241428; E17, AF081330; coxsackie B virus [CBV] 2, AF081312; E26, AJ241433; E27, AF081338; E1, AJ241422; E8, AF081325; E4, AF081319; E21, AF081334; E30, AF081340; E25, AF081336; E29, AJ241434; CBV5, A

Figure. Phylogenetic analysis of complete VP1 sequences of Spanish enterovirus (EV) isolates (GenBank accession nos. DQ468137–DQ468142), the new proposed EV75 sequences (AY556063–AY556070 and AY919545), and prototype EV sequences (echovirus [E] 5, AJ241425; E31, AJ241435; E2, AF081315; E15, AJ241429; E14, AJ241428; E17, AF081330; coxsackie B virus [CBV] 2, AF081312; E26, AJ241433; E27, AF081338; E1, AJ241422; E8, AF081325; E4, AF081319; E21, AF081334; E30, AF081340; E25, AF081336; E29, AJ241434; CBV5, AF114383; CBV6, AF081313; E13, AF081327; EV69, AF081349; E24, AJ241432; E33, AF081346; E3, AF081316; E12, X77708; CBV3, M16572; CBV1, M16560; E6, AF081322; coxsackie A virus [CAV] 9, D00627; E16, AY302542; E9, AF524866; E7, AJ241426; E32, AF081345; E19, AJ241430; E11, AF081326; CBV4, X05690; E18, AF081331; E20, AJ241431; EV70, D17602; CAV6, AF081297; CAV13, AF081303; EV74, AY208118). Phylogenetic trees were constructed with the neighbor-joining method (MEGA version 3.0, available from http://www.megasoftware.net) with Kimura 2-parameter substitution model. Significance of phylogenies was estimated by bootstrap analysis with 1,000 pseudoreplicate datasets. Closed and open circles show Spanish and previously reported EV75 isolates, respectively.

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