Emerging Infectious Disease ISSN: 1080-6059

Chikungunya Virus Strains, Reunion Island Outbreak

Maël Bessaud*, Christophe N. Peyrefitte*†, Boris A.M. Pastorino*, Fabienne Tock*, Olivier Merle*, Jean-Jacques Colpart‡, Jean-Sébastien Dehecq§, Romain Girod*, Marie-Christine Jaffar-Bandjee¶, Pamela J. Glass#, Michael Parker#, Hugues J. Tolou*, and Marc Grandadam*

Author affiliations: *Institut de médecine tropicale du Service de santé des armées, Marseille, France; †Walter Reed Army Institute of Research, Silver Spring, Maryland, USA; ‡Agence de la biomédecine, Lyon, France; §Direction régionale des affaires sanitaires et sociales, Saint-Denis, Île de la Réunion, France; ¶Centre hospitalier départemental Félix-Guyon, Saint-Denis, Île de la Réunion, France; #United States Army Medical Research Institute for Infectious Diseases, Fort Detrick, Maryland, USA

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Figure

Phylogenetic tree of chikungunya virus (CHIKV) based on partial nucleotide sequences (3´ extremity of E1/3´ untranslated, position 10238–11367). Phylogram was constructed with MEGA 2 (http://megasoftware.net/mega2.html), and the tree was drawn with the Jukes-Cantor algorithm for genetic distance determination and the neighbor-joining method. The percentage of successful bootstrap replicates (1,000 bootstrap replications, confidence probability >90%) is indicated at nodes. The length of branch

Figure. Phylogenetic tree of chikungunya virus (CHIKV) based on partial nucleotide sequences (3´ extremity of E1/3´ untranslated, position 10238–11367). Phylogram was constructed with MEGA 2 (http://megasoftware.net/mega2.html), and the tree was drawn with the Jukes-Cantor algorithm for genetic distance determination and the neighbor-joining method. The percentage of successful bootstrap replicates (1,000 bootstrap replications, confidence probability >90%) is indicated at nodes. The length of branches is proportional to the number of nucleotide changes (percentage of divergence). Asterisks (*) indicate strains isolated in this study. The dark triangle corresponds to viruses of the Democratic Republic of Congo clustering together (GenBank accession nos. AY549575–AY549583). O'nyong-nyong virus (ONNV) sequence has been introduced for correct rooting of the tree.

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