Emerging Infectious Disease ISSN: 1080-6059

Distinct Transmission Cycles of Leishmania tropica in 2 Adjacent Foci, Northern Israel

Milena Svobodova*, Jan Votypka*, Jitka Peckova*, Vít Dvorak*, Abedelmajeed Nasereddin†, Gad Baneth†, Julia Sztern†, Vasiliy Kravchenko†, Amnon Orr‡, David Meir§, Lionel F. Schnur†, Petr Volf*, and Alon Warburg†

Author affiliations: *Charles University, Prague, Czech Republic; †The Hebrew University of Jerusalem, Jerusalem, Israel; ‡Tiberias Veterinary Center, Tiberias, Israel; §Nature and National Parks Protection Authority, Jerusalem, Israel

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Figure 2

A) PCR of Leishmania internal transcribed spacer region 1 (ITS1) of naturally infected Phlebotomus sergenti sand flies and cultured Leishmania spp. controls. B) HaeIII digestion of restriction fragment length polymorphisms of ITS1 PCR products shown in A. Lane 1, P. sergenti female 1; lane 2, P. sergenti female 2; lane 3, P. sergenti female 3; lane 4, L. tropica (Lt-L590); lane 5, L. major (Lm-L777); lane 6, L. infantum (Li-L699).

Figure 2. A) PCR of Leishmania internal transcribed spacer region 1 (ITS1) of naturally infected Phlebotomus sergenti sand flies and cultured Leishmania spp. controls. B) HaeIII digestion of restriction fragment length polymorphisms of ITS1 PCR products shown in A. Lane 1, P. sergenti female 1; lane 2, P. sergenti female 2; lane 3, P. sergenti female 3; lane 4, L. tropica (Lt-L590); lane 5, L. major (Lm-L777); lane 6, L. infantum (Li-L699).

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