Figure 2. A) PCR of Leishmania internal transcribed spacer region 1 (ITS1) of naturally infected Phlebotomus sergenti sand flies and cultured Leishmania spp. controls. B) HaeIII digestion of restriction fragment length polymorphisms of ITS1 PCR products shown in A. Lane 1, P. sergenti female 1; lane 2, P. sergenti female 2; lane 3, P. sergenti female 3; lane 4, L. tropica (Lt-L590); lane 5, L. major (Lm-L777); lane 6, L. infantum (Li-L699).