Distinct Transmission Cycles of Leishmania tropica in 2 Adjacent Foci, Northern Israel
Milena Svobodova*, Jan Votypka*, Jitka Peckova*, Vít Dvorak*, Abedelmajeed Nasereddin†, Gad Baneth†, Julia Sztern†, Vasiliy Kravchenko†, Amnon Orr‡, David Meir§, Lionel F. Schnur†, Petr Volf*, and Alon Warburg†
Author affiliations: *Charles University, Prague, Czech Republic; †The Hebrew University of Jerusalem, Jerusalem, Israel; ‡Tiberias Veterinary Center, Tiberias, Israel; §Nature and National Parks Protection Authority, Jerusalem, Israel
Figure 5. Left, female sand fly midgut lysates separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and blotted onto nitrocellulose membranes. Blots were incubated with biotinylated Helix pomatia agglutinin (HPA) that detects O-glycosylated proteins. Lane 1, molecular mass markers; lanes 2 and 3, Phlebotomus sergenti; lanes 4 and 5, P. arabicus; +, preincubation of lectin with 250 mmol/L N-acetyl-d-galactosamine; –, no preincubation. Right, reaction of fluorescein-conjugated HPA with P. arabicus and P. sergenti midgut cells.
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