Emerging Infectious Disease ISSN: 1080-6059
Volume 12, Number 12—December 2006
Dispatch
Spongiform Encephalopathy in a Miniature Zebu
, Catherine Botteron*1, Christian Wenker†, Valeria Café-Marçal*, Anna Oevermann*, Bianca Haase*, Tosso Leeb*, Dagmar Heim‡, and Andreas Zurbriggen*Figure 1
![Histopathologic and immunohistochemical analyses. A) Spongiform lesions; B) partially proteinase K–resistant prion protein (PrPsc) deposits detected by immunohistochemistry (monoclonal antibodies [MAb] F99/97.6.1 diluted 1:500) in the nucleus of the solitary tract (STN) in the zebu under investigation. C–E) Comparative immunohistochemistry with MAb P4 (1:800) in the olivary nuclei of the zebu (C), a bovine spongiform encephalopathy (BSE)-positive cow (D), and a scrapie-positive sheep (E). Insets](/eid/images/06-0750-F1.jpg)
Figure 1. Histopathologic and immunohistochemical analyses. A) Spongiform lesions; B) partially proteinase K–resistant prion protein (PrPsc) deposits detected by immunohistochemistry (monoclonal antibodies [MAb] F99/97.6.1 diluted 1:500) in the nucleus of the solitary tract (STN) in the zebu under investigation. C–E) Comparative immunohistochemistry with MAb P4 (1:800) in the olivary nuclei of the zebu (C), a bovine spongiform encephalopathy (BSE)-positive cow (D), and a scrapie-positive sheep (E). Insets show control tissue slides of BSE-negative cattle. F–H) Immunohistochemistry for PrPsc in lymphoid tissue of the zebu (H, mediastinal lymph node), and a BSE-negative cow (G, mandibular lymph node) with MAb L42 (R-biopharm, 1:800). A retropharyngeal lymph node of a scrapie-affected sheep (F) and a brainstem tissue slide of the zebu (F, inset) served as positive controls. Pretreatment of the tissue slides comprised a proteinase K–digestion step (5 μg/mL, 15 min, 37°C).
1These authors contributed equally to this work.
Lessons from the History of Quarantine, from Plague to Influenza A
Length: 23:11
