Volume 12, Number 2—February 2006
Perspective
Detecting Hepatitis B Surface Antigen Mutants
Paul F. Coleman*
Table 2
Detection of hepatitis B surface antigen (HBsAg) mutants by 4 commercial assays (30)
| Capture/detection | Abbott AxSYM HBsAg mono/poly | Bayer Centaur HBsAg mono/mono | Ortho Vitros ECi HBsAg mono/mono | Roche Elecsys HBsAg mono/mono | |
|---|---|---|---|---|---|
| Recombinant samples | (s/co) | (s/co) | (s/co) | (s/co) | |
| Wild-type | 5.45 | 15.17 | 15.95 | 9.77 | |
| Thr126- Ser | 4.74 | 20.17 | 12.50 | 8.64 | |
| Gln129- His | 4.48 | 20.12 | 13.85 | 8.61 | |
| Met133- Leu | 4.84 | 12.72 | 12.15 | 8.58 | |
| Asp144- Ala | 3.65 | 6.47 | 0.12 | 6.55 | |
| Gly145- Arg | 3.85 | <0.10 | 0.06 | 0.56 | |
| Thr126- Ser + Gly145- Arg | 3.36 | <0.10 | 0.05 | 0.51 | |
| Pro142- Leu + Gly145- Arg | 3.77 | <0.10 | 0.05 | 0.55 | |
| Pro142- Ser + Gly145- Arg | 4.08 | <0.10 | 0.06 | 0.54 | |
| Asp144- Ala + Gly145- Arg | 3.62 | <0.10 | 0.06 | 0.52 | |
| Clinical samples | |||||
| Gly145 - Arg | 5.85 | <0.10 | 0.40 | 0.70 | |
| Pro120-Gln/Thr-131Lys/Gly145-Arg | 2.48 | <0.10 | 0.14 | 0.62 | |
| Thr118-Val/Met133-Ile/Phe134-Asn/Pro142-Ser/Thr143-Leu/Gly145-Arg | 17.60 | <0.10 | 0.11 | 0.67 | |
| Thr115-Asn/Pro120-Leu/Met133-Ile/Phe134-His/Asp144-Val/Ser154-Pro | 2.73 | <0.10 | 0.10 | 0.58 | |
