Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

Volume 12, Number 2—February 2006

Dispatch

Sequencing and Staphylococci Identification

Alexander Mellmann*Comments to Author , Karsten Becker*, Christof von Eiff*, Ursula Keckevoet*, Peter Schumann†, and Dag Harmsen*
Author affiliations: *University Hospital Münster, Münster, Germany; †Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany

Main Article

Table 1

Primers used for amplification and partial sequencing of the partial staphylococcal RNA polymerase B (rpoB) gene

Primer Application Primer sequence (5´→3´) Annealing temperature (°C) Reference
Staph rpoB 1418f* Amplification and sequencing CAA TTC ATG GAC CAA GC 52 Modified from 7
Staph rpoB 3554r Amplification CCG TCC CAA GTC ATG AAA C 52 7
Staph rpoB 1975r* Sequencing GCI ACI TGI TCC ATA CCT GT 52 Modified from 7
Staph rpoB 1876r*† Sequencing GAG TCA TCI TTY TCT AAG AAT GG 52 This study

*Primers are numbered from the 3´ end of the primer on the forward strand of Staphylococcus aureus (GenBank accession no. X64172).
†Primer was used for sequencing when primer Staph rpoB 1975r did not work.

Main Article

TOP