Volume 12, Number 2—February 2006
Sequencing and Staphylococci Identification
|Primer||Application||Primer sequence (5´→3´)||Annealing temperature (°C)||Reference|
|Staph rpoB 1418f*||Amplification and sequencing||CAA TTC ATG GAC CAA GC||52||Modified from 7|
|Staph rpoB 3554r||Amplification||CCG TCC CAA GTC ATG AAA C||52||7|
|Staph rpoB 1975r*||Sequencing||GCI ACI TGI TCC ATA CCT GT||52||Modified from 7|
|Staph rpoB 1876r*†||Sequencing||GAG TCA TCI TTY TCT AAG AAT GG||52||This study|
*Primers are numbered from the 3´ end of the primer on the forward strand of Staphylococcus aureus (GenBank accession no. X64172).
†Primer was used for sequencing when primer Staph rpoB 1975r did not work.
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