Emerging Infectious Disease ISSN: 1080-6059

Identifying and Quantifying Genotypes in Polyclonal Infections due to Single Species

James M. Colborn*, Ousmane A. Koita†, Ousmane Cissé†, Mamadou W. Bagayoko†, Edward J. Guthrie‡, and Donald J. Krogstad*

Author affiliations: *Tulane University Health Sciences Center, New Orleans, Louisiana, USA; †University of Bamako, Bamako, Mali; ‡Agilent Technologies, Wilmington, Delaware, USA

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Figure 2

Copy numbers for genotypes of the K1 allotype in 10 field samples. Distribution of K1 genotypes within the 8 patients whose samples yielded amplicons with K1-specific primers (Table 4). These results indicate that most infected persons had >2 allotypes. In addition, persons with K1 allotype parasites had a high degree of genotypic complexity, that is, capillary electrophoresis showed up to 4 distinct K1 genotypes in the blood of individual patients at the same time.

Figure 2. Copy numbers for genotypes of the K1 allotype in 10 field samples. Distribution of K1 genotypes within the 8 patients whose samples yielded amplicons with K1-specific primers (Table 4). These results indicate that most infected persons had >2 allotypes. In addition, persons with K1 allotype parasites had a high degree of genotypic complexity, that is, capillary electrophoresis showed up to 4 distinct K1 genotypes in the blood of individual patients at the same time.

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