Volume 12, Number 3—March 2006
Research
Identifying and Quantifying Genotypes in Polyclonal Infections due to Single Species
James M. Colborn*, Ousmane A. Koita†, Ousmane Cissé†, Mamadou W. Bagayoko†, Edward J. Guthrie‡, and Donald J. Krogstad*
Table 3
Copy number for amplification of msp1 allotypes in field samples*
| Allotype |
||||
|---|---|---|---|---|
| Field sample | MAD20 | RO33 | K1 | Hybrid |
| 1 | 1,380 | 0 | 15,300 | 467,000 |
| 2 | 0 | 361,000 | 342 | 0 |
| 3 | 813 | 0 | 25.53 | 0 |
| 4 | 138.4 | 0 | 261.4 | 0 |
| 5 | 25.65 | 0 | 1,090 | 101 |
| 6 | 6.71 | 0 | 323.7 | 29.7 |
| 7 | 143 | 0 | 22.6 | 75.7 |
| 8 | 548 | 0 | 361 | 0 |
| 9 | 717 | 0 | 2,320 | 11.9 |
| 10 | 2,330 | 226 | 44.6 | 178 |
*Distribution of allotype-specific copy numbers for samples from 10 subjects infected with Plasmodium falciparum. Both MAD20 and K1 allotype parasites were present in 8 of the 10 subjects, MAD/RO hybrid allotypes were present in 4, and RO33 was present in 1.
