Emerging Infectious Disease ISSN: 1080-6059

Discrimination between Highly Pathogenic and Low Pathogenic H5 Avian Influenza A Viruses

Sunchai Payungporn*, Salin Chutinimitkul*, Arunee Chaisingh†, Sudarat Damrongwantanapokin†, Bandit Nuansrichay†, Wasana Pinyochon†, Alongkorn Amonsin*, Ruben O. Donis‡, Apiradee Theamboonlers*, and Yong Poovorawan*

Author affiliations: *Chulalongkorn University, Bangkok, Thailand; †National Institute of Animal Health, Bangkok, Thailand; ‡Centers for Disease Control and Prevention, Atlanta, Georgia, USA

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Figure

Discrimination between highly pathogenic avian influenza (HPAI) and low pathogenic avian influenza (LPAI) by melting curve analysis based on real-time reverse transcription–polymerase chain reaction of the H5 HA gene with SYBR Green I fluorescent dye. The melting peaks of HPAI and LPAI were clearly separated. The cutoff value was set at 78.50°C (midpoint between HPAI and LPAI) and used to interpret the pathogenicity of unknown samples.

Figure. Discrimination between highly pathogenic avian influenza (HPAI) and low pathogenic avian influenza (LPAI) by melting curve analysis based on real-time reverse transcription–polymerase chain reaction of the H5 HA gene with SYBR Green I fluorescent dye. The melting peaks of HPAI and LPAI were clearly separated. The cutoff value was set at 78.50°C (midpoint between HPAI and LPAI) and used to interpret the pathogenicity of unknown samples.

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